Fig. 2. Polarity of actin filaments in asters induced by treatment with cytochalasin as visualized in platinum replica after decoration with myosin S1. Aster-like aggregate of several actin bundles displays uniform polarity of actin with pointed ends away from, barbed ends toward the center. Arrow indicates tip of filament bundle coming from another actin aster (out of the field of view). Filaments in this bundle also exhibited pointed end away orientation with respect to the center of their aster. Bar, 100 nm.