Figure 2

Fig. 2. MTs remain stationary during axonal growth on Con A-coated substrate. Phase contrast (A-B) and fluorescent (C-D) images of neurite 12 hours after cell culture preparation. The time after photobleaching is indicated in min:sec in the upper right hand corner of each panel. The bleached zone was clearly visible during the experiment (30 min). No obvious change in the position of the bleached segment was observed. The neurite continued to grow and extended by about 20 µm. Bar, 10 µm. (E) Fluorescence intensity profiles of axonal segment including photobleached segment created from images C (20 s after photobleaching, open circles) and D (30 min 20 s after photobleaching, filled squares). The bottom of the fluorescence intensity profile (arrows) remained stationary within experimental error (about 1 µm). Bars, 10 µm.