Figure 7

Fig. 7. Minus-end shortening revealed by sequential subtraction analysis. Subtraction of sequential fluorescence images revealed white (shortening) segments moving in a centrosome-to cell margin direction. These segments, interpreted as minus end shortening, are colorized with red overlay (two MTs highlighted). Length of red segment reflects instantaneous velocity (1.2 µm segment/frame = 10 µm/ min shortening velocity). Elapsed time in seconds shown at lower left; bar 5, µm.