Figure 5

Fig. 5. Analysis of MT dynamics at steady-state. (A) Life history plot of an individual MT at the cell margin. MT end grows and shortens but the net displacement is close to zero. (B) Frequency histograms of plus end displacements after 5 s and 50 s time intervals. The displacement frequency distribution gradually broadened but showed a mean value of approximately zero. Data presented from time-lapse series, 100 images, 5 s intervals; 4410 measurements on 46 MTs in a single cell. (C) Drift component of the random walk. Regression line indicates that drift coefficient is close to zero (-0.04+-0.41 µm/min). (D) Diffusion component of the random walk. Regression line gives value for diffusion coefficient of 7.5+-1.2 µm2/min. Dashed lines, 70% confidence intervals. Data shown, averaged for 5 cells (See text for details).