Fig. 3 Correlative light and electron microscopy of microtubules. REF-52 fibroblast was microinjected with Cy5-tubulin and, after light microscopy, processed for actin removal, then electron microscopy. (a), fluorescence microscopy of a living cell; (b), enlarged boxed region from (a); Copyright © , electron microscopy of the region shown in (b). Microtubules visualized as fluorescent filaments in (a) and (b) can be seen in Copyright © as thick filaments (arrows) within the cytoskeletal network. For better visualization microtubules are highlighted in red. They retain the same pattern as in light microscopic images. Bars, 0.3 µm