Fig. 1 Preparation of culture dishes for correlative microscopy. A circular 18 mm hole was drilled in the bottom of a 35-mm plastic culture dish and a square coverslip (22x22 mm) was mounted onto the bottom with silicone vacuum grease (left). The coverslip was previously coated with gold through a finder grid to create a pattern (right), which is visible in both light and electron microscopy. Cells on clear areas are used for examination.