Fig. 2. Cytoskeletal organization of locomoting and stationary keratocyte fragments. Distribution of myosin II (red) revealed by antibody staining and that of actin (cyan) revealed by staining with tetramethylrhodamine-phalloidin is shown for (a), locomoting fragment and (e), stationary fragment. Electron microscopy of platinum replicas reveals (c), crisscross actin network at the leading edge, and (d), a bundle at the trailing edge of the locomoting fragment (overview is shown in (b)), and (g), a narrow actin brush (indicated with brackets) at the edge of a stationary fragment (overview shown in (f)). A cluster of myosin II minifilaments revealed after extraction of actin from the cytoskeleton of a similar fragment is shown in the inset in (d) at an approximate position that it would occupy in the intact cytoskeleton. (h) Triple staining for actin (cyan), myosin (red) and tubulin (yellow) in several fragments reveals the presence of a few or no microtubules in the fragments. Bars, 2 mm in (a,b,e,f,h), and 200 nm in (c,d,g).
