Fig. 1. Morphology and motility of keratocyte cytoplasmic fragments. (a) Representative images of intact cells. (b) Induction of fragment formation after addition of staurosporine to 100 nM. Low magnification view of developing lamellar fragments (arrows); time in min. See Sequence 1 (1.8 M). (c) Fragments with stalks connecting them to the cell body and (d), fragments disconnected from the cell body; polarized locomoting fragment at bottom of picture, non-polarized stationary one at top (observed in continuous presence of 100 nM staurosporine). (e) Fragment motility plots showing stability and interconvertibility of polarized and non-polarized forms. One fragment (polarized) locomoted smoothly for 40 minutes; another polarized fragment stopped and became non polarized but then repolarized after collision with a different locomoting fragment. Bars, 50 µm in (a), and 10 µm in (b,c,d).
