Figure 4

Fig. 4. Proportion of polymerized tubulin in cytoplasts. (A) Fluorescence images of MTs in cytoplasts with (left) and without (right) the centrosome before (top row) and after (bottom row) extraction into a MT stabilizing buffer. MTs remained stable after lysis but soluble tubulin was extracted. Bars, 10 µm. (B) Proportion of polymerized tubulin was determined ratiometrically on individual cytoplasts as integrated fluorescence after extraction divided by integrated fluorescence in the living state (13). Measurements were performed after flat­fielding and background subtraction. Results presented are for 13 centrosome­containing and 19 centrosome­free cytoplasts.