Figure 8

Fig. 8. Localization of XAC in Xenopus keratocytes. (a-e) Fluorescence microscopy of a whole cell (a-c) and the intensity profile (d) of the enlarged lamellipodium (e) from the boxed region in (c) double stained with XAC antibody (green) and TRITC-phalloidin (red). XAC in lamellipodium is excluded from the narrow zone at the extreme leading edge. (f) Phase contrast sequence of a locomoting cell; time is shown in min. (g-i) Immunoelectron microscopy with XAC antibody of the cell shown in (f), which was lysed and processed at 8 min time point. (g) Cell overview; (h) intermediate magnification of the boxed region from (g) showing distribution of gold particles (yellow) in lamellipodia; (i) high magnification of the boxed region from (d) showing leading edge. XAC is excluded from the extreme front of the lamellipodium (h, i). Bars, 10 µm (a, f); 1 µm (h).