Figure 3

Fig. 3. Localization of Arp2/3 complex in lamellipodia. (a-f) Fluorescence microscopy of Xenopus keratocyte (a-c) or fibroblast (e-g) stained with p21 antibody (green) and TRITC-phalloidin (red) shows Arp 2/3 complex highly enriched in lamellipodia. Boxed region in (g) is enlarged in insets; it shows several filopodia lacking and only one filopodium containing Arp2/3 complex. (d, h) Immunoelectron microscopy of lamellipodia of Xenopus keratocyte (d) or fibroblast (h) stained with p21 primary antibody and 10 nm gold-conjugated secondary antibody after glutaraldehyde fixation and SDS treatment of detergent-extracted cells. Gold particles are highlighted in yellow. Bars, 10 µm (a, e), 0.1 µm (d, h).