Figure 2

Fig. 2. Improved visualization of actin filament branching in lamellipodia. Electron microscopy of keratocyte or fibroblast lamellipodial actin network after CD treatment (0.2 µM, 30 min or 0.5 µM, 10 min) (a, b), 1 min recovery from serum starvation of a mouse fibroblast (c), LA treatment (0.2 µM, 10 min), or unprotected extraction (d). All examples demonstrate frequent branching of actin filaments. Bars, 0.1 µm; (b) and (d) are shown at the same magnification as (c).