Figure 10

Fig. 10. Localization of XAC in Xenopus fibroblasts. (a-e) Fluorescence microscopy of a cell fragment (a-c) and the intensity profile (d) of the enlarged lamellipodium (e) from the boxed region in (c) double stained with XAC antibody (green) and TRITC-phalloidin (red). XAC is distributed throughout the entire lamellipodium. (f, g) Phase contrast sequence showing protruding lamellipodium of a motile cell (f) and the overview of the same cell (g) at 80 sec time point. (h, i) Immunoelectron microscopy with XAC antibody of the cell shown in (g). (h) Cell overview; nucleus and surrouning regions look bright because of high content of cellular proteins which creates high electron density (brightness in inverted contrast); immunogold labelling in these central regions is very low; (i) high magnification of the protruding region pointed to by arrow in (h). Gold particles (yellow) revealing XAC localization are found at the extreme leading edge as well as throughout the lamellipodia.