Protocols

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	Preparation of Cy3-tubulin
	Preparation of rhodamine-tubulin
	Electron Microscopy of Cytoskeleton
	Introduction Cell Culture and Light Microscopy Electron Microscopy Extraction Actin Depletion S1 Decoration Fixation Immunostaining Critical Point Drying Metal Coating Preparation of Replicas Conclusions Acknowledgements Footnotes
	Conjugation of fluorophores to cytoskeletal proteins
	Introduction Preparation of labeled tubulin Microtubule protein preparation Removal of microtubule associated proteins Cy3 labeling of tubulin Preparation of labeled actin Purification of skeletal muscle actin Isolation of actin Fluorescent labeling of actin Preparation of labeled myosin Purification of smooth muscle myosin Fluorescent labeling of smooth muscle myosin

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Last updated: 7/11/2005 Copyright © Laboratory of Molecular Biology 2005