Here is a list of all available supplemental movies for publications. In order to view them, you need the QuickTime movie player. For a more detailed description of the movie, or for more information on the subject, please click on the name of the corresponding article.

• Microinjection of Fluorophore-labeled Proteins : Chapter 5 of Komarova Y., J. Peloquin, and G. Borisy. 2005. Microinjection of Fluorophore–labeled Proteins In Live Cell Imaging: A Laboratory Manual. Robert D. Goldman, David L. Spector, editors, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 67-87.
  1. Sequence 5.1 (216 K) - Microinjection of COS-7 cells into cytoplasm
  2. Sequence 5.1A (460 K) - Microinjection of COS-7 cells into cytoplasm
  3. Sequence 5.2 (512 K) - Microinjection of COS-7 cells into nucleus
  4. Sequence 5.2A (1.6 M) - Microinjection of COS-7 cells into nucleus
  5. Sequence 5.3 (676 K) - Incorporation of fluorophore-labeled tubulin into microtubules
  6. Sequence 5.4 (728 K) - MT dynamics in CHO-K1 cells
  7. Sequence 5.5 (2.3 M) - MT dynamics in CHO-K1 cytoplasts with and without the centrosome
  8. Sequence 5.6 (172 K) - "Speckle" analysis of treadmilled MTs
  9. Sequence 5.7 (88 K) - Dynamics of noncentrosomal MTs in PtK-1 cell
  10. Sequence 5.8 (148 K) - Formation of noncentrosomal MTs in PtK-1 cell
  11. Sequence 5.9 (908 K) - Dynamics of actin in a locomoting fish keratocyte
• SRNA KD down of CP
  1. Sequence 1 (1.8 M) - CP-depletion in B16 mouse melanoma cells: Target 1
  2. Sequence 2 (2.3 M) - CP-depletion in B16 mouse melanoma cells: Target 2
• Arp2/3 Complex and ADF/Cofilin in Dendritic Organization and Treadmilling of Actin Filament Array in Lamellipodia
  1. Sequence 1 (673 K) - Two types of crawling motion
  2. Sequence 2 (1.7 M) - Actin in lamellipodium
  3. Sequence 3 (626 K) - Latrunculin-treated keratocytes
  4. Sequence 4 (711 K) - Actin in latrunculin-treated keratocytes
  5. Sequence 5 (1.2 M) - ADF/cofilin in Xenopus keratocytes
  6. Sequence 6 (1 M) - ADF/cofilin in Xenopus fibroblasts
• Centrosomal Control of Microtubule Dynamics
  1. Sequence 1 (859 K) - MT dynamics in cytoplast with centrosome
  2. Sequence 2 (866 K) - Release from the centrosome, minus end shortening
  3. Sequence 3 (904 K) - Treadmilling in cytoplast without centrosome
  4. Sequence 4 (815 K) - Treadmilling: three of the MTs colored red
  5. Sequence 5 (1.8 M) - "Speckles" in cytoplast without centrosome
• Self-Polarization and Directional Motility of Cytoplasm
  1. Sequence 1 (1.8 M) - Corresponds to Figure 1b. Low magnification view of a field containing both freely locomoting keratocytes and an edge of epithelioid keratocyte colony.
  2. Sequence 2 (192 K) - Corresponds to Figure 3. Dynamics of speckles of injected fluorescent actin analog in a locomoting keratocyte fragment.
  3. Sequence 3 (1 M) - Corresponds to Figure 4a. Dynamics of microinjected myosin II analog in a locomoting keratocyte fragment.
  4. Sequence 4 (480 K) - Corresponds to Figure 4b. Dynamics of microinjected myosin II analog in a stationary keratocyte fragment.
  5. Sequence 5 (2.3 M) - Corresponds to Figure 5. Stationary keratocyte fragment was stimulated by a media stream from a wide micropipette tip.
  6. Sequence 6 (1.3 M) - Corresponds to Figure 7. Myosin II dynamics in an initially stationary keratocyte fragment which polarized after cutting of its stalk with glass microneedle (just before the start of the sequence).
• Transport and Turnover of Microtubules in Frog Neurons Depend on the Pattern of Axonal Growth
  1. Sequence 1 (800 K) - Anterograde movement of photobleached MTs in elongating Xenopus neurite growing on laminin-coated substrate.
  2. Sequence 2 (544 K) - MTs remain stationary during axonal growth on Con A-coated substrate.
  3. Sequence 3 (640 K) - MTs remain stationary in rapidly growing neurites plated on Con A-coated surface in the presence of neurotrophic factors.
• Functional Coordination of Microtubule and Actin Based Motility in Melanophores
  1. Sequence 1 (452 K) - Motility of individual pigment granules in a granule-depleted melanophore fragment with fluorescently labeled microtubules.
  2. Sequence 2 (2 M) - Latrunculin treatment of a cell with dispersed pigment.
  3. Sequence 3 (1.3 M) - Redispersion of pigment granules in a control cell.
  4. Sequence 4 (1.1 M) - Redispersion of pigment granules in lantrunculin-treated cell.
  5. Sequence 5 (531 K) - Pigment aggregation in latrunculin-treated cell.
  6. Sequence 6 (938 K) - Pigment redispersion in the cell shown in Sequence 5.
• Cytoplasmic assembly of microtubules in cultured cells.
  1. Sequence 1 (1 M)
  2. Sequence 2 (2.1 M)
  3. Sequence 3 (0.9 M)
  4. Sequence 4 (2.5 M)
  5. Sequence 5 (1.8 M)
  6. Sequence 6 (1.6 M)
• Keratocytes: Mechanism of Cell Body Translocation.
  1. Sequence 1 (1 M) - Dynamics of microinjected tetramethylrhodamine-myosin II in a locomoting keratocyte.
  2. Sequence 2 (1 M) - Similar to sequence 1 but taken at a higher magnification.
  3. Sequence 3 (1.9 M) - Dynamics of microinjected tetramethylrhodamine-myosin II in a keratocyte tethered at the edge of an epithelioid colony.
  4. Sequence 4 (736 K) - Dynamics of myosin II at the lamellipodium/cell body transition zone of a locomoting keratocyte at high magnification.
• Polarity Sorting of Actin Filaments in Cytochalasin-Treated Fibroblasts.
  1. Sequence 1 (704 K) - Dynamics of microinjected tetramethylrhodamine-myosin II in polarized REF-52 cell treated with cytochalasin D (2 µM).
  2. Sequence 2 (638 K) - Dynamics of microinjected tetramethylrhodamine-myosin II in radially spreading REF-52 cell treated with cytochalasin D.
  3. Sequence 3 (1 M) - Dynamics of microinjected actin labeled with 5-(and 6)-carboxytetramethylrhodamine succinimidyl ester in cytochalasin D-treated REF-52 cell.
  4. Sequence 4 (1 M) - Similar to sequence 3, but actin asters formed in the lamellum showing diffuse distribution of actin prior to addition of cytochalasin.
• Microtubule Release from the Centrosome.
  1. Sequence 1 (671 K) - This sequence includes the one case in which we saw breakage of a MT; this seems to have been caused by stress on the MT.
  2. Sequence 2 (624 K) - Covers 3.5 min beginning at 2.5 min after injection of fluorescent tubulin.
  3. Sequence 3 (1.5 M) - In this sequence one can see released MTs move away from the centrosome, out of the nuclear region, and continue to move about in the cytoplasm.
  4. Sequence 4 (537 K) - A laser was used to create a reference mark on the labeled microtubules by photobleaching; the mark moves with the released MTs, indicating that the entire MT moves, rather than the plus end growing and the minus end shortening (i.e. treadmilling).
  5. Sequence 5 (1.1 M) - Besides the marked MTs, many unmarked MTs (which arose after laser bleaching) are seen to be released and move away from the centrosome.
• Self Centering Activity of Cytoplasm.
  1. Sequence 1 (2.8 M) - Pattern of pigment motion in nascent fragment.
  2. Sequence 2 (2.5 M) - Pattern of pigment motion in nascent fragment after treatment with taxol.
  3. Sequence 3 (1.7 M) - Pigment motion in incubated fragment.
  4. Sequence 4 (1.7 M) - Pigment motion in incubated fragment treated with taxol.
  5. Sequence 5 (1.8 M) - Pigment motion in toroidal fragment.
• Microtubule treadmilling in vivo.
  1. Sequence 1 (1.1 M) - Fish melanophores translocate pigment granules to the center (aggregation) or to the periphery (redispersion) along a radial array of microtubules (MTs).
  2. Sequence 2 (875 K) - Movement of MTs could be achieved either by transport (MT dependent motors bound to a cytoplasmic matrix) or by treadmilling (polymerization at the plus end and depolymerization at the minus end).
  3. Sequence 3 (974 K) - This movie shows another example of bleaching experiment (see the legend to Fig. 2).
  4. Sequence 4 (2.4 M) - This movie shows another example of bleaching experiment (see the legend to Fig. 2).
• Myosin II filament assemblies in the active lamella of fibroblasts: Their morphogenesis and role in the formation of actin filament bundles.
  1. Sequence 1 (1.3 M) - Myosin Streaming.
  2. Sequence 2 (810 K) - More Myosin Streaming.

Last updated: 7/1/2005 Copyright © Laboratory of Molecular Biology 2005